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Catalog Number	Size	Price
LS-C662121-10	10 µg	$318
LS-C662121-100	100 µg	$470

THY1 / CD90 Antibody - Immunohistochemistry - Anti-CD90/Thy1 Picoband Antibody

THY1 / CD90 Antibody - Western blot analysis of CD90/Thy1 using anti-CD90/Thy1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: rat thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD90/Thy1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD90/Thy1 at approximately 26KD. The expected band size for CD90/Thy1 is at 18KD.

THY1 / CD90 Antibody - Western blot - Anti-CD90/Thy1 Picoband Antibody

THY1 / CD90 Antibody - Flow Cytometry analysis of Neuro-2a cells using anti-CD90/Thy1 antibody. Overlay histogram showing Neuro-2a cells stained with anti-CD90/Thy1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD90/Thy1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

THY1 / CD90 Antibody - Flow Cytometry analysis of YAC-1 cells using anti-CD90/Thy1 antibody. Overlay histogram showing YAC-1 cells stained with anti-CD90/Thy1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD90/Thy1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

THY1 / CD90 Antibody - Flow Cytometry analysis of SP2-0 cells using anti-CD90/Thy1 antibody. Overlay histogram showing SP2-0 cells stained with anti-CD90/Thy1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD90/Thy1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Polyclonal Rabbit anti‑Mouse THY1 / CD90 Antibody (IHC, WB) LS‑C662121

Name: Anti-THY1 / CD90 Antibody | Rabbit anti-Mouse Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C662121-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

THY1 / CD90 Rabbit anti-Mouse Polyclonal Antibody

Application:

IHC, IHC-P, IHC-Fr, ICC, WB, Flo

Reactivity:

Mouse, Rat

Format:

Unconjugated, Unmodified

$318

LS-C662121-10

Toll Free North America

(800) 227-6666

For Research Use Only

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Overview

Antibody:

THY1 / CD90 Rabbit anti-Mouse Polyclonal Antibody

Application:

IHC, IHC-P, IHC-Fr, ICC, WB, Flo

Reactivity:

Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

CD90 antibody LS-C662121 is an unconjugated rabbit polyclonal antibody to CD90 (THY1) from mouse. It is reactive with mouse and rat. Validated for Flow, ICC, IHC and WB.

Target

Mouse THY1 / CD90

Synonyms

THY1 | CD90 antigen | CD90 | CDw90 | Thy-1 antigen | Thy-1 T-cell antigen | Thy-1 | Thy-1 cell surface antigen | Thy-1 membrane glycoprotein

See All THY1 / CD90 Antibodies

Host

Rabbit

Reactivity

Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E.coli-derived mouse CD90/Thy1 recombinant protein (Position: Q20-C131). Mouse CD90/Thy1 shares 63.4% and 81.3% amino acid (aa) sequence identity with human and rat CD90/Thy1, respectively.

Applications

IHC
IHC - Paraffin
IHC - Frozen
ICC
Western blot
Flow Cytometry

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About THY1 / CD90

Uniprot: P04216 NCBI: NM_006288 NP_006279.2