Rat THY1 / CD90

Mouse

Rat (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

OX-7

Unconjugated

Protein G purified

Low Endotoxin

Rat Thy1 antigen

Recognizes rat and mouse CD90, also known as Thy1.1, expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (mouse), immature B cells (rat) and connective tissues. Since Thy1.1 is a monomorphic determinant in rat but polymorphic in mice, clone MRC Reacts with Thy1.1 mice e. g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. The affinity of the Fab of MRC OX-7 for rat Thy1 is 3 x 109m-1 and for mouse Thy1.1 is 3 x 108m-1. Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic rat neurons, using fluorescence microscopy. This product is routinely tested in flow cytometry on rat thymocytes.

• IHC - Frozen
• Immunofluorescence
• Western blot
• Immunoprecipitation
• Flow Cytometry (1:40 - 1:80)

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

PBS

Store at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P04216 NCBI: NM_006288 NP_006279.2