LSBio logo with text
Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Location

Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers


Customer Service: (800) 227-6666 / (650) 697-3600


Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > THBS2 / Thrombospondin 2 > LS-C312578
 
Print Page  
Catalog Number Size Price
LS-C312578-10 10 µg $318 
LS-C312578-100 100 µg $470 
THBS2 / Thrombospondin 2 Antibody - THBS2 / Thrombospondin 2 antibody. IHC(P): Human Placenta Tissue.
THBS2 / Thrombospondin 2 Antibody - Western blot analysis of Thrombospondin 2/THBS2 using anti-Thrombospondin 2/THBS2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombospondin 2/THBS2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Thrombospondin 2/THBS2 at approximately 160-190KD. The expected band size for Thrombospondin 2/THBS2 is at 130KD.
THBS2 / Thrombospondin 2 Antibody - Western blot analysis of Thrombospondin 2/THBS2 using anti-Thrombospondin 2/THBS2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 22RV1 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombospondin 2/THBS2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Thrombospondin 2/THBS2 at approximately 160-190KD. The expected band size for Thrombospondin 2/THBS2 is at 130KD.
THBS2 / Thrombospondin 2 Antibody - WB of THBS2 / Thrombospondin 2 antibody. Lane 1: SMMC Cell Lysate. Lane 2: MCF-7 Cell Lysate. Lane 3: HT1080 Cell Lysate. Lane 4: JURKAT Cell Lysate.
THBS2 / Thrombospondin 2 Antibody - Flow Cytometry analysis of U-87MG cells using anti-Thrombospondin 2/THBS2 antibody. Overlay histogram showing U-87MG cells stained with anti-Thrombospondin 2/THBS2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Thrombospondin 2/THBS2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
THBS2 / Thrombospondin 2 Antibody - THBS2 / Thrombospondin 2 antibody. IHC(P): Human Placenta Tissue.
THBS2 / Thrombospondin 2 Antibody - Western blot analysis of Thrombospondin 2/THBS2 using anti-Thrombospondin 2/THBS2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombospondin 2/THBS2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Thrombospondin 2/THBS2 at approximately 160-190KD. The expected band size for Thrombospondin 2/THBS2 is at 130KD.
THBS2 / Thrombospondin 2 Antibody - Western blot analysis of Thrombospondin 2/THBS2 using anti-Thrombospondin 2/THBS2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 22RV1 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombospondin 2/THBS2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Thrombospondin 2/THBS2 at approximately 160-190KD. The expected band size for Thrombospondin 2/THBS2 is at 130KD.
THBS2 / Thrombospondin 2 Antibody - WB of THBS2 / Thrombospondin 2 antibody. Lane 1: SMMC Cell Lysate. Lane 2: MCF-7 Cell Lysate. Lane 3: HT1080 Cell Lysate. Lane 4: JURKAT Cell Lysate.
THBS2 / Thrombospondin 2 Antibody - Flow Cytometry analysis of U-87MG cells using anti-Thrombospondin 2/THBS2 antibody. Overlay histogram showing U-87MG cells stained with anti-Thrombospondin 2/THBS2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Thrombospondin 2/THBS2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
1 of 5
2 of 5
3 of 5
4 of 5
5 of 5

Polyclonal Rabbit anti‑Human THBS2 / Thrombospondin 2 Antibody (aa637‑655, IHC, WB) LS‑C312578

Polyclonal Rabbit anti‑Human THBS2 / Thrombospondin 2 Antibody (aa637‑655, IHC, WB) LS‑C312578

(4.0) Rating
Submit Review (1) Publication(s) LSBio Guarantee
Note: This antibody replaces LS-C389298
Antibody:
THBS2 / Thrombospondin 2 Rabbit anti-Human Polyclonal (aa637-655) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312578-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
THBS2 / Thrombospondin 2 Rabbit anti-Human Polyclonal (aa637-655) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Thrombospondin 2 antibody LS-C312578 is an unconjugated rabbit polyclonal antibody to human Thrombospondin 2 (THBS2) (aa637-655). Validated for IHC and WB. Cited in 1 publication.
(1) Publication(s)
Target
Human THBS2 / Thrombospondin 2
Synonyms
THBS2 | Thrombospondin 2 | TSP2 | Thrombospondin-2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Thrombospondin 2(637-655 aa EAAKTEKQVCEPENPCKDK), different from the mouse sequence by two amino acids.
Epitope
aa637-655
Specificity
High expression in invertebral disk tissue. .
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About THBS2 / Thrombospondin 2
P35442 NM_003247 NP_003238.2

LSBio Ratings

THBS2 / Thrombospondin 2 Antibody (aa637-655) for IHC, WB/Western LS-C312578 has an LSBio Rating of
(4.0) Rating
Publications (4)

Publications (1)

Isolation and Characterization of primary human trabecular meshwork cells from segmental flow regions: New tools for understanding segmental flow. Julia Staverosky, Kamesh Dhamodaran, Ted Acott, VijayKrishna Raghunathan, Janice Vranka. Experimental eye research. 2020 Aug;;197:108046.
Full Text Article PubMed: 32376472 PMC: PMC7483402
Submit a Publication for LS-C312578

Customer Reviews (0)

Submit a review for LS-C312578

Featured Products

Product
THBS2 / Thrombospondin 2 Antibody (aa19‑1172) LS‑C132440
Species: Human
Applications: ELISA
THBS2 / Thrombospondin 2 Antibody - Western blot analysis of Thrombospondin 2 expression in A549 (A); Raw264.7 (B); PC12 (C) whole cell lysates.
THBS2 / Thrombospondin 2 Antibody (C‑Terminus) LS‑C358359
Species: Human, Mouse, Rat, Bovine, Dog, Chicken
Applications: Western blot
THBS2 / Thrombospondin 2 Antibody - Immunohistochemistry of paraffin-embedded human stomach tissue.
THBS2 / Thrombospondin 2 Antibody LS‑C410103
Species: Human, Mouse, Rat
Applications: IHC, Western blot
THBS2 / Thrombospondin 2 Antibody - Western blot of THBS2 / Thrombospondin 2 antibody.
THBS2 / Thrombospondin 2 Antibody (aa968‑1172) LS‑C373195
Species: Human
Applications: Western blot
THBS2 / Thrombospondin 2 ELISA Kit
Rat THBS2 / Thrombospondin 2 (Sandwich ELISA) ELISA Kit - LS-F6601
Reactivity: Rat
Range: 0.78-50 ng/ml
THBS2 / Thrombospondin 2 ELISA Kit
Porcine THBS2 / Thrombospondin 2 (Sandwich ELISA) ELISA Kit - LS-F48492
Reactivity: Porcine
Range: 0.31-20 ng/ml
See all THBS2 / Thrombospondin 2 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/29/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2024 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

To place an order:
customerservice@vectorlabs.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com