Human TGFB1 / TGF Beta 1

Mouse

Human, Monkey, Mouse (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Protein G purified

Unmodified

Hi-5 Insect cell derived recombinant Human TGF-beta1.

Human TGF-beta1

• Western blot (2 - 4 µg/ml)

Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 5 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. *Additional Immunostaining data available. Please contact Tech Support for information. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 4-8 ug/ml of this antibody will detect at least 100 pg/ml of recombinant Human TGF-beta1 when used in conjunction with compatible secondary reagents. Western Blot: To detect Human TGF-beta1 by Western Blot analysis this antibody can be used at a concentration of 2-4 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human TGF-beta1 is 2-4 ng/lane, under non-reducing conditions. In a dot blot, a 1:500 dilution of this antibody exhibited complete cross reactivity with Human TGF-beta2 and TGF-beta3 isotypes. Reduced cross reactivity was shown at a 1:1000 dilution.

Lyophilized from PBS

Reconstitute with sterile water

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01137 NCBI: NM_000660 NP_000651.3