Human TGFA / TGF Alpha

Mouse

Human, Bovine, Dog, Horse, Rabbit, Sheep, Zebrafish (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

P/T1

Unconjugated

Protein G purified

Azide-free, BSA-free. Also available Unmodified.

Synthetic peptide (PPVAAAVVSH, aa34-43 of UniProt P01135) from the human protein. Percent identity by BLAST analysis: Human, Ferret, Sheep, Cat, Bovine, Elephant, Rabbit, Horse, Dog (100%); Mouse, Rat, Hamster, Pig, Opossum, Guinea pig, Chicken, Xenopus (90%); Capsaspora, Shewanella (80%).

aa34-43

Human TGFA / TGF Alpha

• IHC
• IHC - Paraffin (2 - 4 µg/ml)
• Immunofluorescence (1 - 2 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this mAb is completely blocked by the peptide used for raising this antibody. TGFa is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. Optimal dilution of the TGF-alpha antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

1X PBS

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01135 NCBI: NM_003236 NP_003227.1