Human TG / Thyroglobulin

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

SPM221

Unconjugated

Protein G purified

Azide-free, BSA-free. Also available Unmodified.

Human thyroid follicular cells were used as the immunogen for this anti-Thyroglobulin antibody.

Human TG / Thyroglobulin

• IHC - Paraffin (0.1 - 0.2 µg/ml)

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Thyroglobulin is a 660kDa dimeric pre-protein with mutiple glycosylation sites. It is produced by and processed within the thyroid gland to produce the hormone thyroxine and triiodothyronine. Prior to forming dimers, thyroglobulin monomers undergo conformational maturation in the endoplasmic reticulation. The vast majority of follicular carcinomas of the thyroid will give positive immunoreactivity for anti-thyroglobulin even though sometimes only focally. Poorly differentiated carcinomas of the thyroid are frequently anti-thyroglobulin negative. Adenocarcinomas of other-than-thyroid origin do not react with this antibody. This antibody is useful in identification of thyroid carcinoma of the papillary and follicular types. Presence of thyroglobulin in metastatic lesions establishes the thyroid origin of tumor. Anti-thyroglobulin, combined with anti-calcitonin, can identify medullary carcinomas of the thyroid. Furthermore, anti-thyroglobulin, combined with anti-TTF1, can be a reliable marker to differentiate between primary thyroid and lung neoplasms. The optimal dilution of the anti-Thyroglobulin antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

1X PBS

Store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01266 NCBI: NM_003235 NP_003226.4