Target
Baculovirus TERF2 / TRF2
Synonyms
TERF2 | TRF2 | TRBF2 | TTAGGG repeat-binding factor 2 | Telomeric DNA-binding protein
Reactivity
Baculovirus
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein G purified
Immunogen
His-tagged, fusion-protein, corresponding to full-length TRF2 (TTAGGG Repeat binding Factor 2), expressed in baculovirus.
Specificity
Recognizes TRF2, Mr 66kD. Species cross-reactivity: Human and rat.
Applications
- ICC
- Western blot (0.5 - 2 µg/ml)
Usage
Suitable for use in Western Blot and has been reported to demonstrate cell nuclear staining in Immunocytochemistry. Western Blot: 0.5-2 ug/ml detects TRF2 from a RIPA lysate of human Jurkat cells. TRF2 may be detected as a doublet in some cell lysates. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-TRF2 (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Presentation
PBS, pH 7.2, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee