Target
Human TCF7L2 / TCG4
Synonyms
TCF7L2 | HMG box transcription factor 4 | HTCF-4 | T-cell factor-4 variant X2 | T-cell factor 4 | Transcription factor 7-like 2 | TCF-4
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
His-tagged fusion protein corresponding to residues 31-331 of human TCF-4.
Specificity
Recognizes human TCF-4 at 66kD in Cos-1 cells expressing transfected TCF-4. Species cross-reactivity: mouse.
Applications
- IHC
- ICC
- Western blot (0.1 - 2 µg/ml)
- Immunoprecipitation
- ELISA
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Usage
Suitable for use in Western Blot, ELISA, Supershift Assay, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Western Blot: 0.1-2 ug/ml. Detected TCF-4 in RIPA lysates of Cos-1 cells expressing transfected TCF-4. Supershift: Reported to supershift TCF-4: DNA complexes in electrophoretic mobility shift assays (EMSA). Immunoprecipitation: Reported to immunoprecipitate TCF-4 from SW620 whole cell lysates as a complex with b-Catenin. Immunocytochemistry/Immunohistochemistry: Reported to show positive immunostaining for TCF-4 in cells fixed with 4% paraformaldehyde/PBS. Positive control: DLD1 (human colorectal adenocarcinoma), Cos-1 cells or HCT116 (human colorectal carcinoma) cells.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee