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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2563-50 | 50 µg (0.5 mg/ml) | $485 |
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IHC‑plus™ Polyclonal Goat anti‑Human TAGLN / Transgelin / SM22 Antibody (aa190‑201, IHC, WB) LS‑B2563
IHC‑plus™ Polyclonal Goat anti‑Human TAGLN / Transgelin / SM22 Antibody (aa190‑201, IHC, WB) LS‑B2563
Note: This antibody replaces LS-C55559, LS-C89081
Antibody:
TAGLN / Transgelin / SM22 Goat anti-Human Polyclonal (aa190-201) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Xenopus
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
TAGLN / Transgelin / SM22 Goat anti-Human Polyclonal (aa190-201) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Xenopus
Format:
Unconjugated, Unmodified
Specifications
Description
SM22 antibody LS-B2563 is an unconjugated goat polyclonal antibody to SM22 (TAGLN / Transgelin) (aa190-201) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
Target
Human TAGLN / Transgelin / SM22
Synonyms
TAGLN | 22 kDa actin-binding protein | SMCC | TAGLN1 | WS3-10 | Transgelin | Transgelin variant 2 | SM22 | Protein WS3-10 | SM22-alpha
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Xenopus
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-MTGYGRPRQIIS, from the C-Terminus of protein sequence according to NP_003177.2.
Epitope
aa190-201
Specificity
Human TAGLN / SM22. NP_003177.2 and NP_001001522.1 represent identical protein.
Applications
- IHC
- IHC - Paraffin (2.5 - 5 µg/ml)
- Western blot (0.01 - 0.03 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2563 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2563 was determined to be 5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TAGLN / Transgelin / SM22
Validation
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
IHC-plus™ TAGLN / Transgelin / SM22 Antibody (aa190-201) for IHC, WB/Western LS-B2563 has an LSBio Rating of
Publications (4.3)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (4)
Proteomic profiling of chronic low-frequency stimulated fast muscle. Donoghue P, Doran P, Wynne K, Pedersen K, Dunn MJ, Ohlendieck K. Proteomics. 2007 7:3417-30.
Sphingosine 1-phosphate induces differentiation of adipose tissue-derived mesenchymal stem cells towards smooth muscle cells. Nincheri P, Luciani P, Squecco R, Donati C, Bernacchioni C, Borgognoni L, Luciani G, Benvenuti S, Francini F, Bruni P. Cellular and molecular life sciences : CMLS. 2009 66:1741-54.
Transforming growth factor-beta1 induces transdifferentiation of myoblasts into myofibroblasts via up-regulation of sphingosine kinase-1/S1P3 axis. Cencetti F, Bernacchioni C, Nincheri P, Donati C, Bruni P. Molecular biology of the cell. 2010 21:1111-24.
Sphingosine 1-phosphate induces differentiation of mesoangioblasts towards smooth muscle. A role for GATA6. Donati C, Marseglia G, Magi A, Serrat S, Cencetti F, Bernacchioni C, Nannetti G, Benelli M, Brunelli S, Torricelli F, Cossu G, Bruni P. PloS one. 2011 6:e20389.
Customer Reviews (0)
Featured Products
Species:
Rat, Human
Applications:
IHC, IHC - Paraffin
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Reactivity:
Human
Range:
0.156-10 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/22/2024
Date Requested: 12/22/2024
