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Antibodies > T Cell Receptor Alpha Constant > LS-C756589
 
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Catalog Number Size Price
LS-C756589-10 10 µg $318 
LS-C756589-100 100 µg $470 
T Cell Receptor Alpha Constant Antibody - Western blot analysis of TCR alpha using anti-TCR alpha antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TCR alpha protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCR alpha antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TCR alpha at approximately 16KD. The expected band size for TCR alpha is at 16KD.
T Cell Receptor Alpha Constant Antibody - Flow Cytometry analysis of JK cells using anti-TCR alpha antibody. Overlay histogram showing JK cells stained with anti-TCR alpha antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TCR alpha Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
T Cell Receptor Alpha Constant Antibody - Flow Cytometry analysis of THP-1 cells using anti-TCR alpha antibody. Overlay histogram showing THP-1 cells stained with anti-TCR alpha antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TCR alpha Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
T Cell Receptor Alpha Constant Antibody - Western blot analysis of TCR alpha using anti-TCR alpha antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TCR alpha protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCR alpha antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TCR alpha at approximately 16KD. The expected band size for TCR alpha is at 16KD.
T Cell Receptor Alpha Constant Antibody - Flow Cytometry analysis of JK cells using anti-TCR alpha antibody. Overlay histogram showing JK cells stained with anti-TCR alpha antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TCR alpha Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
T Cell Receptor Alpha Constant Antibody - Flow Cytometry analysis of THP-1 cells using anti-TCR alpha antibody. Overlay histogram showing THP-1 cells stained with anti-TCR alpha antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TCR alpha Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human T Cell Receptor Alpha Constant Antibody (WB) LS‑C756589

Polyclonal Rabbit anti‑Human T Cell Receptor Alpha Constant Antibody (WB) LS‑C756589

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Antibody:
T Cell Receptor Alpha Constant Rabbit anti-Human Polyclonal Antibody
Application:
WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C756589-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
T Cell Receptor Alpha Constant Rabbit anti-Human Polyclonal Antibody
Application:
WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
T Cell Receptor Alpha Constant antibody LS-C756589 is an unconjugated rabbit polyclonal antibody to T Cell Receptor Alpha Constant from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow and WB.
Target
Human T Cell Receptor Alpha Constant
Synonyms
TRAC
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human TCR alpha recombinant protein (Position: P1-L116).
Specificity
No cross reactivity with other proteins.
Applications
  • Western blot (0.1 - 0.5 µg/ml)
  • Flow Cytometry (1 - 3 µg/10E6 cells)
  • ELISA (0.1 - 0.5 µg/ml)
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About T Cell Receptor Alpha Constant
P01848 X02592 P01848

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
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