Target
Human SUMO3
Synonyms
SUMO3 | SMT3A | SMT3 homolog 1 | Smt3B | SMT3H1 | SUMO-2 | SUMO-3 | Ubiquitin-like protein SMT3A | Ubiquitin-like protein SMT3B
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Immunogen
This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant human SUMO-3 protein.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Applications
- Immunofluorescence
- Western blot (1:500 - 1:2000)
- ELISA (1:4000 - 1:20000)
Usage
This purified polyclonal antibody reacts with human SUMO-3 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates). Other intrinsic bands are readily detectable at lower dilutions. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 11.6 kD in size corresponding to human SUMO-3 by western blotting in the appropriate cell lysate or extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at 4°C prior to restoration. Restore with 0.1 ml of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee