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Catalog Number Size Price
LS-C153976-2 2 ml (70 mg/ml) $417 
Streptavidin Antibody - Rabbit anti Streptavidin and Biotin conjugated Rabbit anti-trypsin inhibitor antibody were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of primary antibody (ON 4 C). Detection shown was using Dylight 649 conjugated Donkey anti rabbit and
Streptavidin Antibody - Rabbit Anti-Streptavidin Antibody - Dot Blot. Dot Blot showing the detection of Streptavidin. A three-fold serial dilution of DyLight 488 Conjugated Streptavidin starting at 200 ng was spotted onto 0.45 um nitrocellulose and blocked in 1% BSA-TTBS (MB-013, diluted to 1X) 30 min at 20°C. Anti-Streptavidin (RABBIT) Antibody (p/n LS-X35533) was incubated in Blocking Buffer for Fluorescent Western Blot (p/n MB-070) at 1:1000 for 1 Hour at 20°C. An HRP Gt-a-Rb secondary antibody was incubated at 1:40000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc 4000 MP.
Streptavidin Antibody - Rabbit anti Streptavidin and Biotin conjugated Rabbit anti-trypsin inhibitor antibody were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of primary antibody (ON 4 C). Detection shown was using Dylight 649 conjugated Donkey anti rabbit and
Streptavidin Antibody - Rabbit Anti-Streptavidin Antibody - Dot Blot. Dot Blot showing the detection of Streptavidin. A three-fold serial dilution of DyLight 488 Conjugated Streptavidin starting at 200 ng was spotted onto 0.45 um nitrocellulose and blocked in 1% BSA-TTBS (MB-013, diluted to 1X) 30 min at 20°C. Anti-Streptavidin (RABBIT) Antibody (p/n LS-X35533) was incubated in Blocking Buffer for Fluorescent Western Blot (p/n MB-070) at 1:1000 for 1 Hour at 20°C. An HRP Gt-a-Rb secondary antibody was incubated at 1:40000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc 4000 MP.
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Polyclonal Rabbit anti‑Streptomyces avidinii Streptavidin Antibody (IHC, WB) LS‑C153976

Polyclonal Rabbit anti‑Streptomyces avidinii Streptavidin Antibody (IHC, WB) LS‑C153976

Antibody:
Streptavidin Rabbit anti-Streptomyces avidinii Polyclonal Antibody
Application:
IHC, WB, ELISA, DB
Reactivity:
Streptomyces avidinii
Format:
Unconjugated, Unmodified
Price
Catalog Number
$417
LS-C153976-2
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
Streptavidin Rabbit anti-Streptomyces avidinii Polyclonal Antibody
Application:
IHC, WB, ELISA, DB
Reactivity:
Streptomyces avidinii
Format:
Unconjugated, Unmodified

Specifications

Description
Streptavidin antibody LS-C153976 is an unconjugated rabbit polyclonal antibody to streptomyces avidinii Streptavidin. Validated for DB, ELISA, IHC and WB.
Target
Streptomyces avidinii Streptavidin
Host
Rabbit
Reactivity
Streptomyces avidinii (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
Streptavidin (Streptomyces avidinii).
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Streptavidin. No cross reactivity occurs against Avidin.
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:20000 - 1:100000)
  • Dot Blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 2 mL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 12/2/2024