Mouse STAT5A

Mouse

Mouse, Human, Rat (tested or 100% immunogen sequence identity)

Human, Rat (at least 90% immunogen sequence identity)

IgG1,k Monoclonal

5F6.F1

Unconjugated

Protein A affinity chromatography

Unmodified

Anti-STAT5 phospho Y694 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.

pTyr694

This antibody is specific for mouse Stat5a protein phosphorylated at Y694. A BLAST analysis was used to suggest cross-reactivity with Stat5a from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Stat5a from other sources has not been determined.

• IHC (20 - 40 µg/ml)
• Immunofluorescence (1:50 - 1:1000)
• Western blot (1:500 - 1:2000)
• ELISA (1:20000)

Phospho STAT5 pY694 monoclonal antibody is suitable for Immunofluorescence microscopy, ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kD in size corresponding to phosphorylated Stat5a protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with mouse Stat5a pY694 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P42229 NCBI: NM_003152 NP_003143.2