Reactivity
Mouse, Human, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Phycoerythrin.
Also available
Unconjugated or conjugated with
APC,
Biotin,
FITC,
HRP.
Immunogen
A peptide corresponding to a conserved amino acid sequence found near the C-Terminus of murine STAT5. The peptide encompasses the conserved C-Terminus tyrosine phosphorylation site (Y-694) of murine STAT5.
Specificity
Reacts specifically with both mouse STAT5a and STAT5b proteins. Can be used to detect both the un-phosphorylated and phosphorylated forms of STAT5. Does not cross-react with other endogenous STAT isoforms. Species cross-reactivity: Human and rat. Lysates Tested: Cell lysates derived from NIH 3T3 cells, A431 cells, MCF-7 cells, HeLa cells, and 293 cells transfected with either a STAT5 expression vector alone or together with a Jak1 expression vector.