Target
Human STAT1
Synonyms
STAT1 | ISGF-3 | STAT91 | CANDF7
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein A purified
Immunogen
GST fusion-protein containing the C-Terminus 36 amino acids of human STAT1a. This sequence is missing in the b isoform.
Specificity
Recognizes the STAT1 protein, Mr 91kD. of human, mouse and rat. Other species cross-reactivity is unknown.
Applications
- Western blot (0.5 - 2 µg/ml)
- Immunoprecipitation
Usage
Suitable for use in Western Blot, Immunoprecipitation. Western Blot: 0.5-2 ug/ml detects STAT1 in RIPA lysates from human Jurkat, mouse CTLL and rat L6 cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-STAT1 (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 mg immunoprecipitated STAT1 from 500 mg of human Jurkat RIPA lysate.
Presentation
0.07 M Tris-Glycine, 0.105 M NaCl, 0.035% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee