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Antibodies > SQSTM1 > LS-C161484
 
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Catalog Number Size Price
LS-C161484-400 400 µl $393 
SQSTM1 Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
SQSTM1 Antibody - Immunofluorescence staining of Autophagy SQSTM1 (p62) Antibody on Methanol-fixed and PFA fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.
SQSTM1 Antibody - Fluorescent image of U251 cells stained with SQSTM1 (p62) antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated SQSTM1 (p62) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). SQSTM1 (p62) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000161011).
SQSTM1 Antibody - Western blot of SQSTM1 (arrow) using rabbit polyclonal Autophagy SQSTM1 (p62) Antibody (C-term ). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SQSTM1 gene (Lane 2) (Origene Technologies).
SQSTM1 Antibody - Western blot of SQSTM1 Antibody (C331) antibody pre-incubated without(lane 1) and with(lane 2) blocking peptide in MCF-7 cell line lysate. SQSTM1 Antibody (C331) (arrow) was detected using the purified antibody.
SQSTM1 Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
SQSTM1 Antibody - Immunofluorescence staining of Autophagy SQSTM1 (p62) Antibody on Methanol-fixed and PFA fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.
SQSTM1 Antibody - Fluorescent image of U251 cells stained with SQSTM1 (p62) antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated SQSTM1 (p62) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). SQSTM1 (p62) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000161011).
SQSTM1 Antibody - Western blot of SQSTM1 (arrow) using rabbit polyclonal Autophagy SQSTM1 (p62) Antibody (C-term ). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SQSTM1 gene (Lane 2) (Origene Technologies).
SQSTM1 Antibody - Western blot of SQSTM1 Antibody (C331) antibody pre-incubated without(lane 1) and with(lane 2) blocking peptide in MCF-7 cell line lysate. SQSTM1 Antibody (C331) (arrow) was detected using the purified antibody.
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Polyclonal Rabbit anti‑Human SQSTM1 Antibody (aa317‑346, IHC, IF, WB) LS‑C161484

Polyclonal Rabbit anti‑Human SQSTM1 Antibody (aa317‑346, IHC, IF, WB) LS‑C161484

(4.1) Rating
Submit Review (2) Publication(s) LSBio Guarantee
Antibody:
SQSTM1 Rabbit anti-Human Polyclonal (aa317-346) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C161484-400
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
SQSTM1 Rabbit anti-Human Polyclonal (aa317-346) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SQSTM1 antibody LS-C161484 is an unconjugated rabbit polyclonal antibody to human SQSTM1 (aa317-346). Validated for IF, IHC and WB. Cited in 2 publications.
(2) Publication(s)
Target
Human SQSTM1
Synonyms
SQSTM1 | A170 | ORCA | OSIL | Paget disease of bone 3 | p60 | p62 | PDB3 | Sequestosome-1 | Ubiquitin-binding protein p62 | Oxidative stress induced like | p62B | ZIP3 | Sequestosome 1 | EBI3-associated protein p60 | EBIAP
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa317-346
Specificity
This SQSTM1 (p62) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-346 amino acids from the C-terminal region of human SQSTM1 (p62).
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SQSTM1
Q13501 NM_003900 NP_003891.1

LSBio Ratings

SQSTM1 Antibody (aa317-346) for IHC, IF/Immunofluorescence, WB/Western LS-C161484 has an LSBio Rating of
(4.1) Rating
Publications (4.1)

Publications (2)

Impaired protein aggregate handling and clearance underlie the pathogenesis of p97/VCP-associated disease. Ju JS, Miller SE, Hanson PI, Weihl CC. The Journal of biological chemistry. 2008 283:30289-99.
PubMed: 18715868 PMC: PMC2573070
Overexpression of the autophagic beclin-1 protein clears mutant ataxin-3 and alleviates Machado-Joseph disease. Nascimento-Ferreira I, Santos-Ferreira T, Sousa-Ferreira L, Auregan G, Onofre I, Alves S, Dufour N, Colomer Gould VF, Koeppen A, Dglon N, Pereira de Almeida L. Brain : a journal of neurology. 2011 134:1400-15.
PubMed: 21478185
Submit a Publication for LS-C161484

Customer Reviews (0)

Submit a review for LS-C161484

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Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/27/2024
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