Target
Human SPRY2 / Sprouty 2
Synonyms
SPRY2 | HSPRY2 | Protein sprouty homolog 2 | Sprouty homolog 2 (Drosophila) | Sprouty 2 | Spry-2 | Sprouty (Drosophila) homolog 2
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
His6X tagged full-length human Sprouty-2 protein.
Specificity
Reacts with human Sprouty-2 protein (38kD). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Applications
- Western blot (1:2000)
- Immunoprecipitation
- ELISA
Usage
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:2000; A 38kD band corresponding to human Sprouty-2 is observed. Detection occurs using HRP Goat anti-Rabbit IgG and ECL. Immunoprecipitation: 20 ul of antibody is used to immunoprecipitate hSpry2 protein from 1 mg of a HeLa cell lysate in 1ml. Incubate the antibody for 2hr with the cell lysate. Immunoprecipitate the complex with Protein G agarose.
Presentation
PBS, pH 7.2, 0.01% Sodium Azide, No stabilizing proteins added
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee