Monoclonal Mouse anti‑Human SPN / CD43 Antibody (clone Bra7G, Azide‑free,BSA‑free, IHC, IF) LS‑C391006
Monoclonal Mouse anti‑Human SPN / CD43 Antibody (clone Bra7G, Azide‑free,BSA‑free, IHC, IF) LS‑C391006
Antibody:
SPN / CD43 Mouse anti-Human Monoclonal (Azide-free, BSA-free) (Bra7G) Antibody
Application:
IHC, IHC-P, IF, Flo
Format:
Unconjugated, Azide-free, BSA-free
Overview
Antibody:
SPN / CD43 Mouse anti-Human Monoclonal (Azide-free, BSA-free) (Bra7G) Antibody
Application:
IHC, IHC-P, IF, Flo
Format:
Unconjugated, Azide-free, BSA-free
Specifications
Description
CD43 antibody LS-C391006 is an unconjugated mouse monoclonal antibody to human CD43 (SPN). Validated for Flow, IF and IHC.
Target
Human SPN / CD43
Synonyms
SPN | GPL115 | Leukosialin | Leukocyte sialoglycoprotein | Galactoglycoprotein | GALGP | Sialophorin | LSN | CD43 | CD43 antigen
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgM,k
Monoclonal
Conjugations
Unconjugated
Purification
PEG purified
Modifications
Azide-free, BSA-free.
Also available
Unmodified or
Ready-to-Use.
Immunogen
Immature pluripotent human leukemia K562 cells were used as the immunogen for the CD43 antibody.
Specificity
Human SPN / CD43
Applications
- IHC
- IHC - Paraffin (0.5 - 1 µg/ml)
- Immunofluorescence (1 - 2 µg/ml)
- Flow Cytometry (0.5 - 1 µg/10E6 cells)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response (By similarity). [UniProt] Optimal dilution of the CD43 antibody should be determined by the researcher. For staining of formalin-fixed tissues, digest sections with Trypsin at 1mg/ml PBS, 15 min at room temp.
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Featured Products
Species:
Rat
Applications:
Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human, Mouse
Applications:
IHC, Western blot
Species:
Human
Applications:
IHC, Western blot, ELISA
Species:
Mouse
Applications:
IHC, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 10/19/2024