Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C482352-50 50 µl (1 mg/ml) $286 
LS-C482352-100 100 µl (1 mg/ml) $321 
LS-C482352-200 200 µl (1 mg/ml) $386 
SPAM1 / PH20 Antibody - Immunofluorescent analysis of SPAM1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
SPAM1 / PH20 Antibody - Western blot analysis of SPAM1 expression in PC3 (A); Raji (B) whole cell lysates.
SPAM1 / PH20 Antibody - Immunofluorescent analysis of SPAM1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
SPAM1 / PH20 Antibody - Western blot analysis of SPAM1 expression in PC3 (A); Raji (B) whole cell lysates.
1 of 2
2 of 2

Polyclonal Rabbit anti‑Human SPAM1 / PH20 Antibody (IF, WB) LS‑C482352

Polyclonal Rabbit anti‑Human SPAM1 / PH20 Antibody (IF, WB) LS‑C482352

Antibody:
SPAM1 / PH20 Rabbit anti-Human Polyclonal Antibody
Application:
ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$286
LS-C482352-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SPAM1 / PH20 Rabbit anti-Human Polyclonal Antibody
Application:
ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PH20 antibody LS-C482352 is an unconjugated rabbit polyclonal antibody to human PH20 (SPAM1). Validated for ICC, IF and WB.
Target
Human SPAM1 / PH20
Synonyms
SPAM1 | Hyaluronidase PH-20 | PH-20 | Sperm surface protein PH-20 | Sperm adhesion molecule 1 | SPAG15 | HYA1 | Hyal-PH20 | HYAL5 | Hyaluronoglucosaminidase PH-20 | PH20
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Recombinant full length human SPAM1.
Specificity
Recognizes endogenous levels of SPAM1 protein.
Applications
  • ICC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SPAM1 / PH20
P38567 NM_003117 NP_003108.2

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/17/2024