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Catalog Number Size Price
LS-C407720-10 10 µg $318 
LS-C407720-100 100 µg $470 
SOX5 Antibody - Western blot analysis of SOX5 using anti-SOX5 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40ug of sample under reducing conditions. All lanes: pig adipose cellsAfter Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX5 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOX5 at approximately 84KD. The expected band size for SOX5 is at 84KD.
SOX5 Antibody - SOX5 antibody, PBSOX5, Western blot. All lanes: Anti SOX5 at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: Rat Testis Tissue Lysate at 50 ug. Lane 3: Rat Brain Tissue Lysate at 50 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Lane 5: A549 Whole Cell Lysate at 40 ug. Predicted band size: 84 kD. Observed band size: 84 kD.
SOX5 Antibody - Western blot analysis of SOX5 using anti-SOX5 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40ug of sample under reducing conditions. All lanes: pig adipose cellsAfter Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX5 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOX5 at approximately 84KD. The expected band size for SOX5 is at 84KD.
SOX5 Antibody - SOX5 antibody, PBSOX5, Western blot. All lanes: Anti SOX5 at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: Rat Testis Tissue Lysate at 50 ug. Lane 3: Rat Brain Tissue Lysate at 50 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Lane 5: A549 Whole Cell Lysate at 40 ug. Predicted band size: 84 kD. Observed band size: 84 kD.
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Polyclonal Rabbit anti‑Human SOX5 Antibody (aa495‑528, WB) LS‑C407720

Polyclonal Rabbit anti‑Human SOX5 Antibody (aa495‑528, WB) LS‑C407720

Antibody:
SOX5 Rabbit anti-Human Polyclonal (aa495-528) Antibody
Application:
WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407720-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SOX5 Rabbit anti-Human Polyclonal (aa495-528) Antibody
Application:
WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
SOX5 antibody LS-C407720 is an unconjugated rabbit polyclonal antibody to SOX5 (aa495-528) from human. It is reactive with human and rat. Validated for WB.
Target
Human SOX5
Synonyms
SOX5 | L-SOX5 | Transcription factor SOX-5
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human SOX5 (495-528 aa EKEKTTLESLTQQLAVKQNEEGKFSHAMMDFNLS), different from the related mouse sequence by two amino acids.
Epitope
aa495-528
Applications
  • Western blot (0.1 - 0.5 µg/ml)
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SOX5
P35711 NM_006940 NP_008871.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/17/2024