Target
Human Soluble Interleukin 4 Receptor Alpha (sIL4Ra)
Reactivity
Human
(tested or 100% immunogen sequence identity)
Purification
Affinity chromatography
Immunogen
CHO cells derived Recombinant Human IL-4 Receptor alpha
Specificity
Recombinant Human IL-4 Receptor alpha
Applications
- Western blot (0.1 - 0.2 µg/ml)
- ELISA (0.25 - 1 µg/ml)
Usage
ELISA: To detect Human sIL-4 Receptor alpha by sandwich ELISA (using 100µl/well), a concentration of 0.25-1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Human sIL-4 Receptor alpha as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human sIL-4 Receptor alpha . Western Blot: To detect Human sIL-4 Receptor alpha by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human sIL-4 Receptor alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Reconstitute with sterile water
Storage
Before reconstitution: Short term: room temperature for up to 1 month, Long term: store between -20°C and -80°C. After reconstitution: Short term: store at 4°C, Long term: aliquot and store between -20°C and -80°C for up to 6 months. Avoid freeze that cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee