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Catalog Number Size Price
LS-C662480-10 10 µg $318 
LS-C662480-100 100 µg $470 
SMARCA4 / BRG1 Antibody - Immunohistochemistry - Anti-BRG1 Picoband antibody
SMARCA4 / BRG1 Antibody - Immunohistochemistry - Anti-BRG1 Picoband antibody
SMARCA4 / BRG1 Antibody - Immunohistochemistry - Anti-BRG1 Picoband antibody
SMARCA4 / BRG1 Antibody - IF analysis of BRG1 using anti-BRG1 antibody BRG1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-BRG1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
SMARCA4 / BRG1 Antibody - IF analysis of BRG1 using anti-BRG1 antibody BRG1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-BRG1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
SMARCA4 / BRG1 Antibody - Western blot - Anti-BRG1 Picoband antibody
SMARCA4 / BRG1 Antibody - Flow Cytometry analysis of U20S cells using anti-BRG1 antibody. Overlay histogram showing U20S cells stained with anti-BRG1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BRG1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
SMARCA4 / BRG1 Antibody - Immunohistochemistry - Anti-BRG1 Picoband antibody
SMARCA4 / BRG1 Antibody - Immunohistochemistry - Anti-BRG1 Picoband antibody
SMARCA4 / BRG1 Antibody - Immunohistochemistry - Anti-BRG1 Picoband antibody
SMARCA4 / BRG1 Antibody - IF analysis of BRG1 using anti-BRG1 antibody BRG1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-BRG1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
SMARCA4 / BRG1 Antibody - IF analysis of BRG1 using anti-BRG1 antibody BRG1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-BRG1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
SMARCA4 / BRG1 Antibody - Western blot - Anti-BRG1 Picoband antibody
SMARCA4 / BRG1 Antibody - Flow Cytometry analysis of U20S cells using anti-BRG1 antibody. Overlay histogram showing U20S cells stained with anti-BRG1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BRG1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human SMARCA4 / BRG1 Antibody (IHC, WB) LS‑C662480

Polyclonal Rabbit anti‑Human SMARCA4 / BRG1 Antibody (IHC, WB) LS‑C662480

Antibody:
SMARCA4 / BRG1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662480-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
SMARCA4 / BRG1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
BRG1 antibody LS-C662480 is an unconjugated rabbit polyclonal antibody to human BRG1 (SMARCA4). Validated for ELISA, IHC and WB.
Target
Human SMARCA4 / BRG1
Synonyms
SMARCA4 | ATP-dependent helicase SMARCA4 | BAF190 | BRG1-associated factor 190A | Brahma protein-like 1 | BRG1 | BRM/SWI2-related gene 1 | HSNF2b | MRD16 | Protein BRG-1 | SNF2-like 4 | SNF2LB | SNF2-BETA | SNF2B | SWI2 | Nuclear protein GRB1 | SNF2 | SNF2L4 | Transcription activator BRG1 | Homeotic gene regulator | Protein brahma homolog 1 | RTPS2 | Sucrose nonfermenting-like 4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
E. coli-derived human BRG1 recombinant protein (Position: Q555-E763).
Specificity
Colocalizes with ZEB1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level).
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMARCA4 / BRG1
P51532 NM_003072 NP_003063.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/11/2024