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Catalog Number Size Price
LS-C161303-400 400 µl $393 
SMAD9 Antibody - Fluorescent confocal image of MCF-7 cell stained with SMAD9 Antibody. MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD9 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SMAD9 immunoreactivity is localized to cytoplasm and nucleus significantly.
SMAD9 Antibody - SMAD9 Antibody western blot of A549 cell line lysates (35 ug/lane). The SMAD9 antibody detected the SMAD9 protein (arrow).
SMAD9 Antibody - Fluorescent confocal image of MCF-7 cell stained with SMAD9 Antibody. MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD9 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SMAD9 immunoreactivity is localized to cytoplasm and nucleus significantly.
SMAD9 Antibody - SMAD9 Antibody western blot of A549 cell line lysates (35 ug/lane). The SMAD9 antibody detected the SMAD9 protein (arrow).
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Polyclonal Rabbit anti‑Human SMAD9 Antibody (aa200‑228, IF, WB) LS‑C161303

Polyclonal Rabbit anti‑Human SMAD9 Antibody (aa200‑228, IF, WB) LS‑C161303

Antibody:
SMAD9 Rabbit anti-Human Polyclonal (aa200-228) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C161303-400
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
SMAD9 Rabbit anti-Human Polyclonal (aa200-228) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SMAD9 antibody LS-C161303 is an unconjugated rabbit polyclonal antibody to human SMAD9 (aa200-228). Validated for IF and WB. Cited in 1 publication.
Target
Human SMAD9
Synonyms
SMAD9 | MAD homolog 9 | SMAD 9 | SMAD8A | SMAD8 | Smad9 | MADH9 | SMAD8B | MADH6 | Mothers against DPP homolog 9 | SMAD family member 9
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa200-228
Specificity
This SMAD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-228 amino acids from the Central region of human SMAD9.
Applications
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMAD9

LSBio Ratings

SMAD9 Antibody (aa200-228) for IF/Immunofluorescence, WB/Western LS-C161303 has an LSBio Rating of
Publications (4)

Publications (1)

Merkel cell polyomavirus activates LSD1-mediated blockade of non-canonical BAF to regulate transformation and tumorigenesis. Donglim Esther Park, Jingwei Cheng, John P McGrath, Matthew Y Lim, Camille Cushman, Selene K Swanson, Michelle L Tillgren, Joao A Paulo, Prafulla C Gokhale, Laurence Florens, Michael P Washburn, Patrick Trojer, James A DeCaprio. Nature cell biology. 2020 May;22:603-615.

Customer Reviews (0)


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Requested From: United States
Date Requested: 11/12/2024