Target
Human SLIT3
Synonyms
SLIT3 | KIAA0814 | MEGF5 | SLIL2 | Slit homolog 3 protein | Slit-3 | Slit (Drosophila) homolog 3 | Slit homolog 3 (Drosophila)
Reactivity
Human, Mouse, Rat, Bovine, Dog
(tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Mouse, Rat, Bovine, Dog, Chicken (at least 90% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 1155-1180 of Human SLIT-3 protein.
Specificity
A BLAST analysis was used to suggest reactivity with this protein from human, rat, dog, bovine and mouse based on 100% homology for the immunogen sequence. Expect cross reactivity with SLIT-3 from chimpanzee and chicken sources, as only a single amino acid residue change is found within the immunogen sequence (93% positive by BLAST). Cross reactivity with SLIT-3 homologues from other sources has not been determined.
Applications
- Western blot (1 µg/ml)
- ELISA (1:2000 - 1:10000)
Usage
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~168 kD corresponding to full length protein as well as multiple bands corresponding to isoforms of SLIT-3 by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee