Target
Rat SLC9A3 / NHE3
Synonyms
SLC9A3 | Na+/H+ exchanger 3 | NHE3 | Sodium/hydrogen exchanger 3 | Na(+)/H(+) exchanger 3 | NHE-3
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Synthetic peptide consisting of 22aa within the cytoplasmic, C-Terminus domain of rat NHE3.
Specificity
Recognizes rat NHE3. No significant sequence homology exist with other NHE isoforms (NHE1, 2, 4 and 5). Species sequence Homology: Mouse: 90%; human: 76%.
Applications
- Western blot (1 - 10 µg/ml)
- ELISA (1:100000)
Usage
Suitable for use in ELISA and Western Blot. Western Blot: 1-10 ug/ml using ECL; A band of ~90kD has been detected in brush border membrane. ELISA: 1:100000 using 50-100 ng control peptide/well.
Presentation
100 mM Tris, pH 7.5, 0.05% Sodium Azide, 40% Glycerol, 0.2% BSA
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee