Target
Rat SLC9A3 / NHE3
Synonyms
SLC9A3 | Na+/H+ exchanger 3 | NHE3 | Sodium/hydrogen exchanger 3 | Na(+)/H(+) exchanger 3 | NHE-3
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Synthetic peptide consisting of 22aa within the cytoplasmic, C-Terminus domain of the rat NHE3 (KLH coupled).
Specificity
Recognizes rat NHE3. Species sequence Homology: Mouse: 90%; Human: 76%.
Applications
- Western blot (1 - 10 µg/ml)
- ELISA (1:100000)
Usage
Suitable for use ELISA and Western Blot. ELISA: 1:100000. Using 50-100 ng control peptide/well. Western blot: 1-10 ug/ml (ECL). A band of ~90kD has been detected in brush border membrane.
Presentation
PBS, pH 7.4, 0.05% Sodium Azide, 0.1% BSA
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee