Target
Rat SLC6A3 / Dopamine Transporter
Synonyms
SLC6A3 | DA transporter | DAT1 | DAT
Reactivity
Rat, Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
An 18 amino acid peptide sequence near the NH2-terminus of rat brain dopamine transporter coupled to KLH (1). This region is presumably the cytoplasmic domain.
Specificity
Recognizes Dopamine transporter (DAT). Species cross-reactivity: Rat and mouse. Weakly reacts with human and canine.
Applications
- IHC
- IHC - Frozen
- Western blot (1 - 10 µg/ml)
- Immunoprecipitation
- ELISA (0.5 - 1 µg/ml)
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Usage
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, and ELISA. Western Blot: 1-10 ug/ml using ECL. An antibody raised against a similar peptide has been shown to react with a broad 80kD band in rat striatum and not cerebellum. Rat DAT contains various carbohydrate moieties and neuraminidase and N-Glycanase treatment reduces its molecular weight to about 55kD. Immunoprecipitation: 2-10 ug. Immunohistochemistry: 2-10 ug/ml on frozen sections of tissues fixed with 4% paraformaldehyde. ELISA: 0.5-1.0 ug/ml using 1 ug/ml of DAT control peptide per well.
Presentation
PBS, pH 7.4, 0.05% Sodium Azide, 0.1% BSA
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SLC6A3 / Dopamine Transporter