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Catalog Number Size Price
LS-C15416-100 100 µg $832 
Antibody

Polyclonal Rabbit anti‑Human SLC2A5 / GLUT5 Antibody (N‑Terminus, IHC, IF, WB) LS‑C15416

Polyclonal Rabbit anti‑Human SLC2A5 / GLUT5 Antibody (N‑Terminus, IHC, IF, WB) LS‑C15416

Antibody:
SLC2A5 / GLUT5 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$832
LS-C15416-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
SLC2A5 / GLUT5 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
GLUT5 antibody LS-C15416 is an unconjugated rabbit polyclonal antibody to human GLUT5 (SLC2A5) (N-Terminus). Validated for ELISA, IF, IHC and WB.
Target
Human SLC2A5 / GLUT5
Synonyms
SLC2A5 | Fructose transporter | Glucose transporter 5 | GLUT-5 | GLUT5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
A cytoplasmic 12 AA peptide sequence near the C-Terminus of human glucose/fructose transporter (Glut-5) (1). A cysteine has been added at the NH2 terminus for coupling to KLH. The peptide was coupled to KLH.
Epitope
N-Terminus
Specificity
Human GT52 sequences shows about 61% homology with rat Glut-5 (8/13 AA). For rat Glut-5, we suggest the use of antibodies that are made to a peptide from rat Glut-5. Antibody cross-reactivity in various species is not established. Control peptide, because of its low mol size (<3kD), is not recommended for Western. It should be used in ELISA, Dot Blot and for antibody blocking to confirm specificity of antibodies.
Applications
  • IHC
  • Immunofluorescence
  • Western blot (1 - 10 µg/ml)
  • ELISA (0.5 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Western Blot: 1-10 ug/ml (ECL). Rat glut-5 is ~60kD in rat jejunum membranes that is slightly larger that that reported for human glut-5 (50-55kD). As opposed to human tissues, rat glut-5 mRNA has not been detected in rat testis, adipose tissues and skeletal muscle. In rat kidney, a protein of ~42kD was detected. ELISA: 0.5-1 ug/ml. Control peptide can be used to coat ELISA plates at 1 ug/ml.
Presentation
PBS, pH 7.4, 0.1% BSA, 0.02% sodium merthiolate, 40% glycerol.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SLC2A5 / GLUT5
P22732 NM_003039 NP_003030.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024