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Catalog Number Size Price
LS-C186488-100 100 µg (0.5 mg/ml) $503 
SLC1A3 / EAAT1 Antibody - SLC1A3 antibody (0.1 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Polyclonal Goat anti‑Human SLC1A3 / EAAT1 Antibody (aa197‑208, WB) LS‑C186488

Polyclonal Goat anti‑Human SLC1A3 / EAAT1 Antibody (aa197‑208, WB) LS‑C186488

Antibody:
SLC1A3 / EAAT1 Goat anti-Human Polyclonal (aa197-208) Antibody
Application:
WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C186488-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
SLC1A3 / EAAT1 Goat anti-Human Polyclonal (aa197-208) Antibody
Application:
WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
EAAT1 antibody LS-C186488 is an unconjugated goat polyclonal antibody to human EAAT1 (SLC1A3) (aa197-208). Validated for Peptide-ELISA and WB.
Target
Human SLC1A3 / EAAT1
Synonyms
SLC1A3 | EA6 | EAAT-1 | EAAT1 | GLAST | GLAST1 | GLAST-1
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-RSFKVPIQANET, from the internal region of the protein sequence according to NP_004163.3NP_001160167.1.
Epitope
aa197-208
Specificity
Human SLC1A3 / GluT-1 / GLAST. This antibody is expected to recognize isoform 1 (NP_004163.3) and isoform 2 (NP_001160167.1).
Applications
  • Western blot (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 60kD band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 59.6kD according to NP_004163.3). Recommended concentration: 0.1-0.3 ug/ml. An additional band of unknown identity was also consistently observed at 22kD. This band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SLC1A3 / EAAT1
P43003 NM_004172 NP_004163.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024