Target
Human SLC1A3 / EAAT1
Synonyms
SLC1A3 | EA6 | EAAT-1 | EAAT1 | GLAST | GLAST1 | GLAST-1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-RSFKVPIQANET, from the internal region of the protein sequence according to NP_004163.3NP_001160167.1.
Specificity
Human SLC1A3 / GluT-1 / GLAST. This antibody is expected to recognize isoform 1 (NP_004163.3) and isoform 2 (NP_001160167.1).
Applications
- Western blot (0.1 - 0.3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 60kD band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 59.6kD according to NP_004163.3). Recommended concentration: 0.1-0.3 ug/ml. An additional band of unknown identity was also consistently observed at 22kD. This band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee