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Catalog Number	Size	Price
LS-C662158-10	10 µg	$318
LS-C662158-100	100 µg	$470

SIX3 Antibody - Western blot analysis of Six3 using anti-Six3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,Lane 2: mouse brain tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Six3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Six3 at approximately 35KD. The expected band size for Six3 is at 35KD.

Polyclonal Rabbit anti‑Human SIX3 Antibody (WB) LS‑C662158

Name: Anti-SIX3 Antibody | Rabbit anti-Human Polyclonal WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C662158-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

SIX3 Rabbit anti-Human Polyclonal Antibody

Application:

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C662158-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

SIX3 Rabbit anti-Human Polyclonal Antibody

Application:

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

SIX3 antibody LS-C662158 is an unconjugated rabbit polyclonal antibody to human SIX3. Validated for WB.

Target

Human SIX3

Synonyms

SIX3 | HPE2 | Sine oculis homeobox homolog 3 | SIX homeobox 3 | Homeobox protein SIX3

See All SIX3 Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

A synthetic peptide corresponding to a sequence at the N-Terminus of human Six3 (1-32 aa MVFRSPLDLYSSHFLLPNFADSHHRSILLASS), different from the related mouse sequence by two amino acids.

Applications

Western blot

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About SIX3

Uniprot: O95343 NCBI: NM_005413 NP_005404.1