Target
Human SIRT2 / Sirtuin 2
Synonyms
SIRT2 | SIR2 | Sir2-related protein type 2 | SIR2L | SIR2L2 | Sirtuin 2 | Silent information regulator 2 | SIR2-like protein 2 | Sirtuin type 2
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified from tissue culture supernatant
Immunogen
Recombinant human Sirtuin 2.
Specificity
Recognizes human sirtuin 2. Detects a band of ~45kDa by Western blot.
Applications
- Western blot (1:2000 - 1:5000)
- ELISA (1:2000 - 1:5000)
Usage
ELISA - Direct and indirect: 1:2000-1:5000. Western Blot - 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature.
Presentation
PBS, pH 7.4, no preservatives.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee