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Catalog Number Size Price
LS-C497222-20 20 µl $275 
LS-C497222-100 100 µl $389 
SIGLEC8 Antibody - Western blot analysis of extracts of various cell lines, using SIGLEC8 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Polyclonal Rabbit anti‑Human SIGLEC8 Antibody (WB) LS‑C497222

Polyclonal Rabbit anti‑Human SIGLEC8 Antibody (WB) LS‑C497222

Antibody:
SIGLEC8 Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C497222-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
SIGLEC8 Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
SIGLEC8 antibody LS-C497222 is an unconjugated rabbit polyclonal antibody to SIGLEC8 from human. It is reactive with human, mouse and rat. Validated for WB.
Target
Human SIGLEC8
Synonyms
SIGLEC8 | CDw329 | CD329 antigen | SAF-2 | SIGLEC-8 | Sialoadhesin family member 2 | SIGLEC8L | SAF2
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 17-363 of human SIGLEC8 (NP_055257.2). MEGDRQYGDGYLLQVQELVTVQEGLCVHVPCSFSYPQDGWTDSDPVHGYWFRAGDRPYQDAPVATNNPDREVQAETQGRFQLLGDIWSNDCSLSIRDARKRDKGSYFFRLERGSMKWSYKSQLNYKTKQLSVFVTALTHRPDILILGTLESGHSRNLTCSVPWACKQGTPPMISWIGASVSSPGPTTARSSVLTLTPKPQDHGTSLTCQVTLPGTGVTTTSTVRLDVSYPPWNLTMTVFQGDATASTALGNGSSLSVLEGQSLRLVCAVNSNPPARLSWTRGSLTLCPSRSSNPGLLELPRVHVRDEGEFTCRAQNAQGSQHISLSLSLQNEGTGTSRPVSQVTLAA
Applications
  • Western blot (1:500 - 1:2000)
Usage
The predicted MW is 44kDa/46kDa/54kDa, while the observed MW by Western blot was 60kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SIGLEC8
Q9NYZ4 NM_014442 NP_055257.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 2/12/2025