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| Catalog Number | Size | Price |
|---|---|---|
| LS-C359327-0.1 | 0.1 ml (1 mg/ml) | $386 |
1 of 2
2 of 2
Polyclonal Rabbit anti‑Human SHH / Sonic Hedgehog Antibody (aa1‑75, IHC, IF, WB) LS‑C359327
Polyclonal Rabbit anti‑Human SHH / Sonic Hedgehog Antibody (aa1‑75, IHC, IF, WB) LS‑C359327
Antibody:
SHH / Sonic Hedgehog Rabbit anti-Human Polyclonal (aa1-75) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Primate
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
SHH / Sonic Hedgehog Rabbit anti-Human Polyclonal (aa1-75) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Primate
Format:
Unconjugated, Unmodified
Specifications
Description
Sonic Hedgehog antibody LS-C359327 is an unconjugated rabbit polyclonal antibody to Sonic Hedgehog (SHH) (aa1-75) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.
Target
Human SHH / Sonic Hedgehog
Synonyms
SHH | HHG-1 | HLP3 | Sonic hedgehog | Sonic hedgehog homolog | MCOPCB5 | Sonic hedgehog protein | HHG1 | HPE3 | SMMCI | TPT | TPTPS
Host
Rabbit
Reactivity
Human, Mouse, Rat, Primate
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide made to an N-Terminus portion of the human SHH protein (between residues 1-75) [UniProt Q15465]
Epitope
aa1-75
Specificity
Human SHH / Sonic Hedgehog
Applications
- IHC
- IHC - Paraffin (1:200 - 1:400)
- ICC (1:200)
- Immunofluorescence (1:200)
- Western blot (1 - 2 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
This SHH antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band is observed at ~50 kDa. In Immunocytochemistry/Immunofluorescence cytoplasm/membrane staining was observed in SH-SY5Y cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About SHH / Sonic Hedgehog
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Featured Products
Species:
Human, Mouse, Chicken
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
Immunofluorescence, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, ICC, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Non-Human Primate
Applications:
IHC, Western blot, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
Date Requested: 11/23/2024
