Target
Human SFRP1
Synonyms
SFRP1 | FrzA | FRP | SARP-2 | SARP2 | SFRP-1 | FRP-1 | FRP1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
NS0-derived rhsFRP-1 (aa31-313).
Specificity
Recognizes human sFRP-1. Shows less than 1% cross-reactivity with rmsFRP-1, rhsFRP-3 and rhsFRP-4.
Applications
- Western blot (0.1 - 0.2 µg/ml)
- ELISA (0.5 - 1 µg/ml)
Usage
Suitable for use in Direct ELISA and Western Blot. Direct ELISA: 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human sFRP-1. The detection limit for rhsFRP-1 is ~0.6 ng/well. Testing was performed using the recombinant antigen as the sample. Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human sFRP-1. The detection limit for rhsFRP-1 is ~5 ng/lane under non-reducing and reducing. conditions.
Presentation
PBS, pH 7.2, 5% Trehalose, 40% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee