Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C313483-10 10 µg $318 
LS-C313483-100 100 µg (0.5 mg/ml) $470 
SFN / Stratifin / 14-3-3 Sigma Antibody - SFN / Stratifin / 14-3-3 Sigma antibody. IHC(P): Human Esophagus Squama Cancer Tissue.
SFN / Stratifin / 14-3-3 Sigma Antibody - SFN / Stratifin / 14-3-3 Sigma antibody. IHC(P): Human Tonsil Tissue.
SFN / Stratifin / 14-3-3 Sigma Antibody - SFN / Stratifin / 14-3-3 Sigma antibody. IHC(P): Human Tonsil Tissue.
SFN / Stratifin / 14-3-3 Sigma Antibody - ICC analysis of 14-3-3 sigma using anti-14-3-3 sigma antibody. 14-3-3 sigma was detected in immunocytochemical section of human Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-14-3-3 sigma Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SFN / Stratifin / 14-3-3 Sigma Antibody - Anti-14-3-3 sigma antibody, Western blotting All lanes: Anti 14-3-3 sigma at 0.5ug/ml Lane 1: HELA Whole Cell Lysate at 40ug Lane 2: A549 Whole Cell Lysate at 40ug Lane 3: A549 Whole Cell Lysate at 40ug Lane 4: COLO320 Whole Cell Lysate at 40ug Lane 5: SE620 Whole Cell Lysate at 40ug Predicted bind size: 28KD Observed bind size: 28KD
SFN / Stratifin / 14-3-3 Sigma Antibody - WB of SFN / Stratifin / 14-3-3 Sigma antibody. Lane 1: HELA Cell Lysate. Lane 2: A549 Cell Lysate. Lane 3: A549 Cell Lysate. Lane 4: COLO320 Cell Lysate. Lane 5: SE620 Cell Lysate.
SFN / Stratifin / 14-3-3 Sigma Antibody - SFN / Stratifin / 14-3-3 Sigma antibody. IHC(P): Human Esophagus Squama Cancer Tissue.
SFN / Stratifin / 14-3-3 Sigma Antibody - SFN / Stratifin / 14-3-3 Sigma antibody. IHC(P): Human Tonsil Tissue.
SFN / Stratifin / 14-3-3 Sigma Antibody - SFN / Stratifin / 14-3-3 Sigma antibody. IHC(P): Human Tonsil Tissue.
SFN / Stratifin / 14-3-3 Sigma Antibody - ICC analysis of 14-3-3 sigma using anti-14-3-3 sigma antibody. 14-3-3 sigma was detected in immunocytochemical section of human Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-14-3-3 sigma Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SFN / Stratifin / 14-3-3 Sigma Antibody - Anti-14-3-3 sigma antibody, Western blotting All lanes: Anti 14-3-3 sigma at 0.5ug/ml Lane 1: HELA Whole Cell Lysate at 40ug Lane 2: A549 Whole Cell Lysate at 40ug Lane 3: A549 Whole Cell Lysate at 40ug Lane 4: COLO320 Whole Cell Lysate at 40ug Lane 5: SE620 Whole Cell Lysate at 40ug Predicted bind size: 28KD Observed bind size: 28KD
SFN / Stratifin / 14-3-3 Sigma Antibody - WB of SFN / Stratifin / 14-3-3 Sigma antibody. Lane 1: HELA Cell Lysate. Lane 2: A549 Cell Lysate. Lane 3: A549 Cell Lysate. Lane 4: COLO320 Cell Lysate. Lane 5: SE620 Cell Lysate.
1 of 6
2 of 6
3 of 6
4 of 6
5 of 6
6 of 6

Polyclonal Rabbit anti‑Human SFN / Stratifin / 14‑3‑3 Sigma Antibody (aa140‑156, IHC, WB) LS‑C313483

Polyclonal Rabbit anti‑Human SFN / Stratifin / 14‑3‑3 Sigma Antibody (aa140‑156, IHC, WB) LS‑C313483

Note: This antibody replaces LS-C357706
Antibody:
SFN / Stratifin / 14-3-3 Sigma Rabbit anti-Human Polyclonal (aa140-156) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Horse, Pig, Rabbit, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313483-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SFN / Stratifin / 14-3-3 Sigma Rabbit anti-Human Polyclonal (aa140-156) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Horse, Pig, Rabbit, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
14-3-3 Sigma antibody LS-C313483 is an unconjugated rabbit polyclonal antibody to 14-3-3 Sigma (SFN / Stratifin) (aa140-156) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IHC and WB.
Target
Human SFN / Stratifin / 14-3-3 Sigma
Synonyms
SFN | 14-3-3 sigma | 14-3-3 protein sigma | HME1 | Stratifin | YWHAS
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Horse, Pig, Rabbit, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human 14-3-3 sigma(140-156 aa KKRIIDSARSAYQEAMD), identical to the related rat and mouse sequences.
Epitope
aa140-156
Specificity
Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SFN / Stratifin / 14-3-3 Sigma
P31947 NM_006142 NP_006133.1

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/29/2024