Human SELPLG / PSGL-1 / CD162

Mouse

Human, Baboon, African green monkey, Cynomolgus monkey, Rhesus monkey (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

KPL-1

Unconjugated

Purified by affinity chromatography.

Unmodified

PSGL-1 transfected murine 300.19 pre B-cell line

• IHC
• IHC - Paraffin (5 - 10 µg/ml)
• Western blot
• Immunoprecipitation
• Flow Cytometry (0.5 µg/10E6 cells)

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.5 µg per million cells in 100 µl volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application. Clone KPL-1 is reported to recognize the tyrosine sulfation consensus motif of PSGL-11. Additional reported applications (for the relevant formats) include: Western Blot1, immunoprecipitation2, immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin embedded tissue sections, blocks the recognition of PSGL-1 with P- and L-selectin1.

PBS pH 7.2, 0.09% sodium azide.

Store undiluted at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q14242 NCBI: NM_003006 NP_002997.2