Coronavirus SARS-CoV SARS-CoV Nucleoprotein

Rabbit

Coronavirus SARS-CoV, Virus (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Protein A purified

Unmodified

This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant protein corresponding to full length SARS Coronavirus Nucleocapsid protein. Lifesensors Inc. (www.lifesensors.com) prepared the Nucleocapsid protein as follows: SUMO-Nucleocapsid fusion was expressed in E.coli in LB medium and purified using Ni-NTA resin (Qiagen) affinity chromatography. After the fusion was cleaved by the SUMO Protease (LifeSensors), the SUMO tag and protease were subtracted from the nucleocapsid using MAC and the nucleocapsid was finally purified using Cation Exchange Chromatography with the Macro-Prep High S resin (BioRad) and size exclusion chromatography.

• Immunofluorescence
• Western blot (1:2000 - 1:10000)
• ELISA (1:10000 - 1:50000)

Suitable for use in ELISA and by Western Blot. Specific conditions for reactivity should be optimized by the end user.

Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Reconstitute in 100 µL of deionized water.

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

NCBI: NP_828858.1