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Catalog Number Size Price
LS-C407889-10 10 µg $318 
LS-C407889-100 100 µg $470 
S100A6 / Calcyclin Antibody - S100 alpha 6 antibody IHC-paraffin. IHC(P): Human Placenta Tissue.
S100A6 / Calcyclin Antibody - IHC analysis of S100A6 using anti-S100A6 antibody. S100A6 was detected in immunocytochemical section of LOVO 1 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-S100A6 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
S100A6 / Calcyclin Antibody - S100 alpha 6 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.
S100A6 / Calcyclin Antibody - S100 alpha 6 antibody IHC-paraffin. IHC(P): Mouse Testis Tissue.
S100A6 / Calcyclin Antibody - S100 alpha 6 antibody IHC-paraffin. IHC(P): Rat Lung Tissue.
S100A6 / Calcyclin Antibody - S100 alpha 6 antibody Western blot. All lanes: Anti S100 alpha 6 at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: A431 Whole Cell Lysate at 40 ug. Predicted band size: 10 kD. Observed band size: 10 kD.
S100A6 / Calcyclin Antibody - Flow Cytometry analysis of A431 cells using anti-S100A6 antibody. Overlay histogram showing A431 cells stained with anti-S100A6 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-S100A6 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
S100A6 / Calcyclin Antibody - Flow Cytometry analysis of SiHa cells using anti-S100A6 antibody. Overlay histogram showing SiHa cells stained with anti-S100A6 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-S100A6 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
S100A6 / Calcyclin Antibody - S100 alpha 6 antibody IHC-paraffin. IHC(P): Human Placenta Tissue.
S100A6 / Calcyclin Antibody - IHC analysis of S100A6 using anti-S100A6 antibody. S100A6 was detected in immunocytochemical section of LOVO 1 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-S100A6 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
S100A6 / Calcyclin Antibody - S100 alpha 6 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.
S100A6 / Calcyclin Antibody - S100 alpha 6 antibody IHC-paraffin. IHC(P): Mouse Testis Tissue.
S100A6 / Calcyclin Antibody - S100 alpha 6 antibody IHC-paraffin. IHC(P): Rat Lung Tissue.
S100A6 / Calcyclin Antibody - S100 alpha 6 antibody Western blot. All lanes: Anti S100 alpha 6 at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: A431 Whole Cell Lysate at 40 ug. Predicted band size: 10 kD. Observed band size: 10 kD.
S100A6 / Calcyclin Antibody - Flow Cytometry analysis of A431 cells using anti-S100A6 antibody. Overlay histogram showing A431 cells stained with anti-S100A6 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-S100A6 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
S100A6 / Calcyclin Antibody - Flow Cytometry analysis of SiHa cells using anti-S100A6 antibody. Overlay histogram showing SiHa cells stained with anti-S100A6 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-S100A6 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human S100A6 / Calcyclin Antibody (aa1‑90, IHC, WB) LS‑C407889

Polyclonal Rabbit anti‑Human S100A6 / Calcyclin Antibody (aa1‑90, IHC, WB) LS‑C407889

Antibody:
S100A6 / Calcyclin Rabbit anti-Human Polyclonal (aa1-90) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407889-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
S100A6 / Calcyclin Rabbit anti-Human Polyclonal (aa1-90) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Calcyclin antibody LS-C407889 is an unconjugated rabbit polyclonal antibody to Calcyclin (S100A6) (aa1-90) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human S100A6 / Calcyclin
Synonyms
S100A6 | 2A9 | 5B10 | CABP | CACY | PRA | MLN 4 | Protein S100-A6 | Calcyclin
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human S100 alpha 6 recombinant protein (Position: M1-G90). Human S100 alpha 6 shares 96.6% and 95.5% amino acid (aa) sequence identity with mouse and rat S100 alpha 6, respectively.
Epitope
aa1-90
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About S100A6 / Calcyclin
P06703 NM_014624 NP_055439.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024