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Catalog Number Size Price
LS-C154980-100 100 µg (0.5 mg/ml) $503 
RRAD Antibody - RRAD antibody a): (0.5 ug/ml) staining of HEK293 lysates overexpressing several HA-tagged Mouse GTPases, including Rrad (10 ug protein in RIPA buffer)  and compared with an HA-specific antibody. B): (0.5 ug/ml) staining of WT and KO lysates of Mouse Heart (100 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Polyclonal Goat anti‑Human RRAD Antibody (aa36‑48, WB) LS‑C154980

Polyclonal Goat anti‑Human RRAD Antibody (aa36‑48, WB) LS‑C154980

Antibody:
RRAD Goat anti-Human Polyclonal (aa36-48) Antibody
Application:
WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C154980-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
RRAD Goat anti-Human Polyclonal (aa36-48) Antibody
Application:
WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
RRAD antibody LS-C154980 is an unconjugated goat polyclonal antibody to RRAD (aa36-48) from human. It is reactive with human, mouse, rat and other species. Validated for Peptide-ELISA and WB.
Target
Human RRAD
Synonyms
RRAD | GTP-binding protein RAD | Ras associated with diabetes | REM3 | RAD
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-HRRSMPVDERDLQ, from the internal region of the protein sequence according to NP_004156.1.
Epitope
aa36-48
Specificity
Human RRAD. Reported variants represent identical protein: NP_004156.1, NP_001122322.1
Applications
  • Western blot (0.5 - 2 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 37kD band observed in Mouse Heart lysates and no 37kD in the KO lysates (calculated MW of 33.2kD according to mouse NP_062636.2 and human NP_004156.1). There is some non-specific background, we call for caution when used for other assays than Western blot. In transfected HEK293 transiently expressing Mouse Rrad (HA tagged), a band of approx. 45kD is observed. No bands are observed in transfected HEK293 transiently expressing related Mouse GTPases. Data provided by Prof. D Andres, University of Kentucky College of Medicine, USA. Recommended concentration: 0.5-2 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RRAD
P55042 NM_004165 NP_004156.1

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Requested From: United States
Date Requested: 12/22/2024