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Catalog Number	Size	Price
LS-C354090-100	100 µl (1 mg/ml)	$315

RPS6KB1 / P70S6K / S6K Antibody - Immunohistochemical analysis of S6K1 (pT444) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

RPS6KB1 / P70S6K / S6K Antibody - Immunofluorescent analysis of S6K1 (pT444) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

RPS6KB1 / P70S6K / S6K Antibody - Western blot analysis of S6K1 (pT444) expression in HepG2 EGF-treated (A); mouse brain (B); rat brain (C) whole cell lysates.

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Polyclonal Rabbit anti‑Human RPS6KB1 / P70S6K / S6K Antibody (phospho‑Thr444, IHC, IF, WB) LS‑C354090

Name: Anti-RPS6KB1 / P70S6K / S6K Antibody | Rabbit anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-C354090-100
Price: 315.0000 USD
Availability: InStock

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Antibody:

RPS6KB1 / P70S6K / S6K Rabbit anti-Human Polyclonal (pThr444) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Chicken

Format:

Unconjugated, Unmodified

$315

LS-C354090-100

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Overview

Antibody:

RPS6KB1 / P70S6K / S6K Rabbit anti-Human Polyclonal (pThr444) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Chicken

Format:

Unconjugated, Unmodified

Specifications

Description

S6K antibody LS-C354090 is an unconjugated rabbit polyclonal antibody to S6K (RPS6KB1 / P70S6K) (pThr444) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.

Target

Human RPS6KB1 / P70S6K / S6K

Synonyms

See All RPS6KB1 / P70S6K / S6K Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human S6K1 (pT444).

Epitope

pThr444

Specificity

Recognizes endogenous levels of S6K1 (pT444) protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence
Western blot (1:500 - 1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About RPS6KB1 / P70S6K / S6K

Uniprot: P23443 NCBI: NM_003161 NP_003152.1