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Antibodies > RPL4 / Ribosomal Protein L4 > LS-C757569
 
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Catalog Number Size Price
LS-C757569-200 200 µl $393 
RPL4 / Ribosomal Protein L4 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling RPL4 with RPL4 Antibody (N-Term) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
RPL4 / Ribosomal Protein L4 Antibody - All lanes: Anti-RPL4 Antibody (N-Term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Raji whole cell lysate Lane 5: NCI-H929 whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
RPL4 / Ribosomal Protein L4 Antibody - Overlay histogram showing U-2OS cells stained with RPL4 Antibody (N-Term) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (RPL4 Antibody (N-Term), 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
RPL4 / Ribosomal Protein L4 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling RPL4 with RPL4 Antibody (N-Term) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
RPL4 / Ribosomal Protein L4 Antibody - All lanes: Anti-RPL4 Antibody (N-Term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Raji whole cell lysate Lane 5: NCI-H929 whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
RPL4 / Ribosomal Protein L4 Antibody - Overlay histogram showing U-2OS cells stained with RPL4 Antibody (N-Term) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (RPL4 Antibody (N-Term), 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Polyclonal Rabbit anti‑Human RPL4 / Ribosomal Protein L4 Antibody (N‑Terminus, IF, WB) LS‑C757569

Polyclonal Rabbit anti‑Human RPL4 / Ribosomal Protein L4 Antibody (N‑Terminus, IF, WB) LS‑C757569

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Antibody:
RPL4 / Ribosomal Protein L4 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IF, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C757569-200
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
RPL4 / Ribosomal Protein L4 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IF, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Ribosomal Protein L4 antibody LS-C757569 is an unconjugated rabbit polyclonal antibody to human Ribosomal Protein L4 (RPL4) (N-Terminus). Validated for ELISA, Flow, IF and WB.
Target
Human RPL4 / Ribosomal Protein L4
Synonyms
RPL4 | 60S ribosomal protein L1 | 60S ribosomal protein L4 | L4 | Ribosomal protein L4 | RPL1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Bovine (at least 90% immunogen sequence identity)
Clonality
Ig Polyclonal
Conjugations
Unconjugated
Purification
Protein A and Peptide Affinity Chromatography
Modifications
Unmodified
Immunogen
This RPL4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 119-149 amino acids from human RPL4.
Epitope
N-Terminus
Applications
  • Immunofluorescence (1:25)
  • Western blot (1:2000)
  • Flow Cytometry (1:25)
  • ELISA
Usage
Western Blot Predicted Molecular Weight: 47.7 kDa
Presentation
PBS, 0.09% Sodium Azide
Storage
Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RPL4 / Ribosomal Protein L4
P36578 NM_000968 NP_000959.2

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Species: Human, Mouse, Rat
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RPL4 / Ribosomal Protein L4 Antibody - RPL4 / L4 antibody Western Blot of Human 721_B lysate. Antibody Dilution: 1.0 ug/ml. RPL4 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells. Antibody dilution: 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
RPL4 / Ribosomal Protein L4 Antibody (C‑Terminus, FITC) LS‑C472226
Species: Human, Chimpanzee, Orangutan, Gibbon, Mouse, Rat, Pig
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Tag: His-GST
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/25/2024
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Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

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