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Catalog Number	Size	Price
LS-C745292-25	25 µl (1 mg/ml)	$304

ROBO1 Antibody - Affinity Purified anti-ROBO1 antibody was used at a concentration of 5 µg/ml to detect ROBO1 in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows staining of human brain tissue. Tissue was formalin-fixed and paraffin embedded.

ROBO1 Antibody - Staining of ROBO1 in undifferentiated, immortalized human podocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 2% paraformaldehyde and 4% sucrose at room temperature for 10 minutes. The cells were then washed once with PBS, permeabilized with 0.3% Triton X-100 for 10 minutes and incubated with blocking solution (2% FCS, 2% BSA, 0.2% fish gelatin) for 30 minutes, before further incubation with primary Ab for 1 hour. An Alexa Fluor 488 goat anti-rabbit IgG secondary antibody was used at a dilution of 1/200. DAPI was used for nuclear counterstaining.

ROBO1 Antibody - 1/50 staining mouse lung tissue sections (adult, frozen 100µm wholemount sections) by IHC-Fr. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4°C.

ROBO1 Antibody - Western Blot of rabbit anti-ROBO1 antibody. Marker: Opal Pre-stained ladder Lane 1: HEK293 lysate Lane 2: HeLa Lysate Lane 3: MCF-7 Lysate Lane 4: Jurkat Lysate Lane 5: A431 Lysate Lane 6: A549 Lysate Lane 7: LNCap Lysate Lane 8: MOLT-4 Lysate Lane 9: Ramos Lysate Lane 10: Raji Lsyate Lane 11: A-172 Lysate Lane 12: NIH/3T3 Lysate Load: 35 µg per lane. Primary antibody: ROBO1 antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 181kDa for ROBO1.

ROBO1 Antibody - Western Blot of rabbit anti-Robo-1 antibody. Lane 1: HeLa WCL. Load: 30 µg per lane. Primary antibody: Robo-1 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 181 kDa, 181 kDa for Robo-1. Other band(s): not identified.

ROBO1 Antibody - ELISA results of purified Rabbit anti-Robo-1 Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins)

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Polyclonal Rabbit anti‑Human ROBO1 Antibody (aa1625‑1650, IHC, IF, WB) LS‑C745292

Name: Anti-ROBO1 Antibody | Rabbit anti-Human Polyclonal ELISA,IF,IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C745292-25
Price: 304.0000 USD
Availability: InStock

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Antibody:

ROBO1 Rabbit anti-Human Polyclonal (aa1625-1650) Antibody

Application:

IHC, IF, WB, ELISA

Reactivity:

Human, Mouse, Rat, Dog

Format:

Unconjugated, Unmodified

$304

LS-C745292-25

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For Research Use Only

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Overview

Antibody:

ROBO1 Rabbit anti-Human Polyclonal (aa1625-1650) Antibody

Application:

IHC, IF, WB, ELISA

Reactivity:

Human, Mouse, Rat, Dog

Format:

Unconjugated, Unmodified

Specifications

Description

ROBO1 antibody LS-C745292 is an unconjugated rabbit polyclonal antibody to ROBO1 (aa1625-1650) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB.

Target

Human ROBO1

Synonyms

See All ROBO1 Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)

Predicted

Mouse, Rat, Dog (at least 90% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Affinity purified

Modifications

Unmodified

Immunogen

This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an C-Terminal region near amino acids 1625-1650 of Human ROBO-1.

Epitope

aa1625-1650

Specificity

A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat and dog sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of ROBO-1. Cross reactivity with ROBO-1 homologues from other sources has not been determined.

Applications

IHC (2 - 10 µg/ml)
Immunofluorescence
Western blot (1:500 - 1:3000)
ELISA (1:30000 - 1:160000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Applications should be user optimized.

Presentation

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Storage

Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About ROBO1

Uniprot: Q9Y6N7 NCBI: NM_002941 NP_002932.1