Target
Bovine Ribonuclease A / RNASE1
Synonyms
RNASE1 | RIB1 | Ribonuclease A | Ribonuclease pancreatic | RNase 1 | RNase UpI-1 | RNS1 | Ribonuclease 1 | RNase A | HP-RNase | RIB-1
Reactivity
Bovine
(tested or 100% immunogen sequence identity)
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Ribonuclease A [Bovine Pancreas].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ribonuclease A [Bovine Pancreas]. Cross reactivity against Ribonuclease A from other sources may occur but have not been specifically determined.
Applications
- Western blot (1:500 - 1:2500)
- Immunoprecipitation (1:100)
- ELISA (1:150000)
Usage
Anti-Ribonuclease A antibody has been assayed against 1.0 ug of Ribonuclease A in a standard capture ELISA using Peroxidase Conjugated Streptavidin ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:3000 to 1:13000 of the reconstitution concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Ribonuclease A / RNASE1