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Catalog Number Size Price
LS-C817117-100 100 µl $412 
RFC4 Antibody - Immunochemical staining of human RFC4 in human small intestine with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
RFC4 Antibody - Immunofluorescence staining of RFC4 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human RFC4 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
RFC4 Antibody - Anti-RFC4 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa.
RFC4 Antibody - RFC4 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 4 uL anti-RFC4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RFC4 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa.
RFC4 Antibody - Immunochemical staining of human RFC4 in human small intestine with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
RFC4 Antibody - Immunofluorescence staining of RFC4 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human RFC4 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
RFC4 Antibody - Anti-RFC4 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa.
RFC4 Antibody - RFC4 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 4 uL anti-RFC4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RFC4 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa.
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Polyclonal Rabbit anti‑Human RFC4 Antibody (IHC, IF, WB) LS‑C817117

Polyclonal Rabbit anti‑Human RFC4 Antibody (IHC, IF, WB) LS‑C817117

Antibody:
RFC4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$412
LS-C817117-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
RFC4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
RFC4 antibody LS-C817117 is an unconjugated rabbit polyclonal antibody to human RFC4. Validated for ICC, IF, IHC, IP and WB.
Target
Human RFC4
Synonyms
RFC4 | Activator 1 subunit 4 | A1 37 kDa subunit | Activator 1 37 kd subunit | Activator 1 37 kDa subunit | RFC37 | Replication factor C subunit 4 | RF-C 37 kDa subunit | RFC 37 kDa subunit
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
E. coli-derived Human RFC4 fragment
Specificity
Human RFC4
Applications
  • IHC - Paraffin (1:50 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.0, 0.03% Proclin 300
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RFC4
P35249 BC024022 NP_002907.1

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Requested From: United States
Date Requested: 11/26/2024