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Vector Laboratories, Inc.
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Customer Service: (800) 227-6666 / (650) 697-3600


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Catalog Number Size Price
LS-C312865-10 10 µg $318 
LS-C312865-100 100 µg (0.5 mg/ml) $470 
RFC1 / RFC Antibody - IHC analysis of RFC1 using anti-RFC1 antibody. RFC1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-RFC1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
RFC1 / RFC Antibody - WB of RFC1 / RFC antibody. Lane 1: HELA Cell Lysate. Lane 2: SMMC Cell Lysate. Lane 3: JURKAT Cell Lysate. Lane 4: CEM Cell Lysate. Lane 5: 293T Cell Lysate.
RFC1 / RFC Antibody - Western blot analysis of RFC1 using anti-RFC1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA Cell Lysate, Lane 2: SMMC Cell Lysate, Lane 3: JURKAT Cell Lysate, Lane 4: CCRF-CEM Cell Lysate, Lane 5: 293T Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFC1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RFC1 at approximately 150KD. The expected band size for RFC1 is at 128KD.
RFC1 / RFC Antibody - IHC analysis of RFC1 using anti-RFC1 antibody. RFC1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-RFC1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
RFC1 / RFC Antibody - WB of RFC1 / RFC antibody. Lane 1: HELA Cell Lysate. Lane 2: SMMC Cell Lysate. Lane 3: JURKAT Cell Lysate. Lane 4: CEM Cell Lysate. Lane 5: 293T Cell Lysate.
RFC1 / RFC Antibody - Western blot analysis of RFC1 using anti-RFC1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA Cell Lysate, Lane 2: SMMC Cell Lysate, Lane 3: JURKAT Cell Lysate, Lane 4: CCRF-CEM Cell Lysate, Lane 5: 293T Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFC1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RFC1 at approximately 150KD. The expected band size for RFC1 is at 128KD.
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Polyclonal Rabbit anti‑Human RFC1 / RFC Antibody (aa46‑64, IHC, WB) LS‑C312865

Polyclonal Rabbit anti‑Human RFC1 / RFC Antibody (aa46‑64, IHC, WB) LS‑C312865

Note: This antibody replaces LS-C389147
Antibody:
RFC1 / RFC Rabbit anti-Human Polyclonal (aa46-64) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312865-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
RFC1 / RFC Rabbit anti-Human Polyclonal (aa46-64) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
RFC antibody LS-C312865 is an unconjugated rabbit polyclonal antibody to RFC (RFC1) (aa46-64) from human. It is reactive with human and monkey. Validated for IHC and WB.
Target
Human RFC1 / RFC
Synonyms
RFC1 | A1 140 kDa subunit | Activator 1 large subunit | Activator 1 140 kDa subunit | Activator 1 subunit 1 | DNA-binding protein PO-GA | Replication factor C subunit 1 | RF-C 140 kDa subunit | MHC binding factor, beta | PO-GA | MHCBFB | RECC1 | Replication factor C1 | RFC | RFC140
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the N-Terminus of human RFC1(46-64 aa NSSRKEDDFKQKQPSKKKR).
Epitope
aa46-64
Specificity
Wide tissue distribution. Undetectable in placental tissue.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RFC1 / RFC
P35251 NM_002913 NP_002904.3

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/4/2024