Mouse Retnla / RELM Alpha

Rabbit

Mouse (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

E.coli derived recombinant Murine RELMalpha.

Mouse RELMalpha

• Western blot (0.1 - 0.2 µg/ml)
• ELISA (0.5 - 2 µg/ml)

Immunohistochemistry: This antibody stained 4% PFA, paraffin-embedded sections of murine cecum tissue (with positive cells infiltrating the Lamina Propia of a Trichuris muris infected mouse). The primary antibody was incubated at 4 ng/mL overnight at 4°C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM Citric Acid was used. Information and photo are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary. *Additional Immunostaining data available. Please contact Tech Support for information. ELISA: To detect Murine RELMalpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine RELMalpha (LS-C104489) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine RELMalpha. Western Blot: To detect Murine RELMalpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELMalpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Lyophilized from PBS, pH 7.2

Reconstitute in sterile water to 0.1-1 mg/ml.

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9EP95 NCBI: NP_065255.2