Human RBP4

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Protein G purified

Unmodified

NS0-derived rhRBP4.

Recognizes human RBP4. Species cross-reactivity: Recombinant mouse RBP4 25%.

• Western blot (1 - 2 µg/ml)
• Flow Cytometry
• ELISA (0.5 - 1 µg/ml)

Suitable for use in Flow Cytometry, ELISA and Western Blot. Western Blot: 1-2 ug/ml. Using a colorimetric detection system, the detection limit for rhRBP4 is ~25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity 5-50 fold. Flow Cytometry (HepG2 cells): 25 ug/ml For intracellular staining to detect RBP4, cells must first be fixed and permeabilized using 4% paraformaldehyde and ice-cold methanol. Add 10 ul of the diluted solution to 1-5x10^5 cells in a total reaction volume not exceeding 200ul. ELISA: 0.5-1 ug/ml. The detection limit for rhRBP4 is ~5 ng/well.

Lyophilized from PBS, pH 7.4, 5% Trehalose

Reconstitute in sterile PBS.

Lyophilized and resuspended forms: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02753 NCBI: NM_006744 NP_006735.2