Target
Human RBM3
Synonyms
RBM3 | IS1-RNPL | RNA binding motif protein 3 | RNPL | RNA-binding motif protein 3 | Putative RNA-binding protein 3
Reactivity
Human, Mouse, Rat, Bovine, Horse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-DRYSGGNYRDNYDN, from the C-Terminus of protein sequence according to NP_006734.1.
Applications
- Western blot (0.01 - 0.03 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:2000)
Usage
Peptide ELISA: antibody detection limit dilution 1:2000. Western blot: Approx.17kDa band observed in lysates of cell line HepG2 (calculated MW of 17.2kDa according to NP_006734.1). Recommended concentration: 0.01-0.03 ug/ml. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee