Target
Human RBFOX3 / NEUN
Reactivity
Human, Mouse, Rat, Bovine
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
N-Terminus 100 amino acids of human Fox3 as expressed in and purified from E. coli. We did not use full length Fox3 as immunogen since the three mammalian Fox homologues, namely Fox1, Fox2 and Fox3, include virtually identical motifs. The N-Terminus of the three molecules are much more variable in the three molecules so antibodies specific for each of the three molecules can therefore be generated.
Specificity
Clone 1B7 is known to react with Fox3/NeuN from human, cow, pig, mouse, rat and other mammals. Since Fox3/NeuN is highly conserved, it is likely that the antibody is effective on other species also.
Applications
- ICC (1:1000)
- Immunofluorescence (1:1000)
- Western blot (1:1000 - 1:2000)
Usage
The antibody solution can be used at dilutions of 1:1000 or higher in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:1000-1:2000.
Presentation
PBS, 10 mM Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee