Target
Mouse RARRES2 / Chemerin
Synonyms
RARRES2 | HP10433 | RAR-responsive protein TIG2 | TIG2 | Chemerin
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
Biotin.
Purification
Protein G purified
Immunogen
E. coli-derived rmChemerin.
Specificity
Selected for its ability to detect mouse Chemerin in direct ELISAs and Western Blots.
Applications
- Western blot (1 - 2 µg/ml)
- ELISA (0.5 - 1 µg/ml)
- Neutralization (1 - 5 µg/ml)
Usage
Suitable for use in Western Blot, Neutralization and ELISA. Western Blot: 1-2 ug/ml. Using a colorimetric detection system, the detection limit is ~10 ng/lane under non-reducing conditions. Chemiluminescent detection will increase sensitivity 5-50 fold. Neutralization: 1-5 ug/ml in the presence of 100 ng/ml of rmChemerin, using the human ChemR23 transfected BaF/3 cell chemotaxis assay. ELISA: 0.5-1.0 ug/ml. Detection limit is ~10 ng/well. Hybridoma: Mouse myeloma with B cells obtained from a rat immunized with purified, E. coli-derived, recombinant mouse Chemerin (rmChemerin; aa17-156; Accession # NP_082128).
Presentation
Lyophilized from PBS, 5% Trehalose
Reconstitution
Reconstitute in sterile PBS.
Storage
Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee